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○ Date : 2015 10 15() 오후 4:30 ~ 5:30
○ Place : 
삼성학술도서관 7
Creative Learning Room
○ Speaker : Dr. Ja Yil Lee
이자일 박사 (콜럼비아 대학교, 미국
)
○ Topic : Single-Molecule Approach to Homology Search & Strand Exchange in Homologous Recombination



Abstract:

Homologous recombination (HR) is an important biological phenomenon because it repairs DNA double-strand break in an error-free way and rearranges genetic information during meiosis promoting genetic diversity. HR is mediated by the Rad51/RecA family of recombinases. The essential roles of the recombinases are searching for the homologous sequence and then exchanging DNA strands. Despite decades of work, the physical basis of the homology search and strand-exchange is not well-defined. Using the single-molecule DNA curtain technique, we find that 8-nt is the minimum size to search for homologous sequences and that the search process is accelerated by the facilitated exchange mechanism meaning by competitor-dependent dissociation. We also reveal that the strand exchange occurs in precise 3-nucleotide (nt) steps for prokaryotic RecA and eukaryotic Rad51 and Dmc1. The free energy difference between the steps is ~0.3 kBT, which is also conserved from bacteria to human. Molecular dynamics simulation suggests that Watson-Crick basepairing of base triplets is the molecular basis of the triplet stepping. Furthermore we show that all the recombinases can step over mismatched bases in the strand exchange. But only the meiosis-specific recombinase Dmc1 can step over mismatched bases without any destabilization of base-triplets, which enhances more genetic exchange during meiosis.



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